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Using these profile HMMs and the HMM search algorithm implemented in HMMER3, remote homologs () for each of the 17 protein families were identified across four independent databases: 1) Phylo Pro (Xiong et al.

We further show a unique structural feature of gametocytes that is defined by a -specific IMC protein, perhaps representing a cytoskeletal innovation required for sexual development in this genus.

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Depending on the ecological niche of the organisms, the function of the IMC ranges from a fundamental role as reinforcement system to more specialized roles in motility and cytokinesis.

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2002) was used to assess the level of phylogenetic signal/conflict in each data.

Double transfectant parasites expressing PF14_0578-m Cherry and Pf Centrin3 were generated by transfecting 100 μg of p B-PF14_0578-m Cherry into the transgenic II-linearized transfection plasmid as previously described (Janse et al.

The 17 resultant MSAs were manually edited to remove any ambiguity using Se-Al v2.0a11 (Rambaut et al.

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